Recombinant:Article Title: Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD206 Abcam Cat# ab64693; RRID: AB_1523910 PD-L1 clone E1L3N Cell Signaling Technology Cat# 13684; RRID: AB_2687655 AffiniPure Fab Fragment Goat Anti-Rabbit IgG (H+L) Jackson Immunology Cat# 111-007-003 Chemicals, peptides, and recombinant proteins Emerald antibody diluent Sigma-Aldrich Cat# 936B-08 Antigen unmasking solution, citrate buffer (1003) Vector Laboratories Cat# H-3300 ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat# H-4000 ImmPress Polymer Reagent anti-Rabbit Vector Laboratories Cat# MP-7451-15 VIP HRP substrate chromogen Vector Laboratories Cat# SK-4600 DPX mounting media Sigma-Aldrich Cat# 6522 Harris hematoxylin Sigma-Aldrich Cat# HHS16 Trizma base Sigma-Aldrich Cat# T1503 NaCl Sigma-Aldrich Cat# S9888 Tween 20 Sigma-Aldrich Cat# P7949 HCL Hydrogen peroxide 30% VWR Cat# 23619.264 PBS (phosphate-buffered saline) (pH 7.4) Gibco Cat# 10010023 DPBS, calcium, magnesium Gibco Cat #14040117 Ethanol absolute (99%) Fisher Scientific Uk Ltd Cat# 10437341 Ammonium hydroxide Sigma-Aldrich Cat# 221228-1L-A Xylene Fisher Scientific Uk Ltd Cat# 10385910 10% neutral buffered formalin CellPath Plc Cat # BAF-0100-25A Dako wash buffer 103 Agilent Cat # S300685-2 Other NanoZoomer S210 Digital slide scanner Hamamatsu Cat# C13239-01 Tefal 6 Liter stainless steel pressure cooker Tefal Cat # 3045384362433 Hot plate Cole-Parmer Cat# 11476508 Microtome Leica Cat# RM2125 RTS Slide jars Simport Cat# M906-12AS Cover slides VWR Cat# 631-0146 IHC slides Thermo Fisher Cat# 12312148 Embedding cassettes Epredia Cat# 16323986 RS PRO Borosilicate Glass 600 mL Beaker RS PRO Cat# 461-1137 Fume hood Fumair Cat# 52716 Staining tray Agar Scientific Cat# AGAR_L4474 Oven (hybridizer hb-1) Techne Cat# 39455-03 Software and algorithms ImageJ FIJI https://imagej.nih.gov/ij/https://fiji.sc/ 1.53f51 QuPath https://qupath.github.io/ 0.2.0m.8 NDP.view2 https://www.hamamatsu.com/us/en/ product/type/U12388-01/index.html 2.7.25 GraphPad https://www.graphpad.com/scientific-software/prism/ 9.0.2 ..
Polymer:Article Title: Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD206 Abcam Cat# ab64693; RRID: AB_1523910 PD-L1 clone E1L3N Cell Signaling Technology Cat# 13684; RRID: AB_2687655 AffiniPure Fab Fragment Goat Anti-Rabbit IgG (H+L) Jackson Immunology Cat# 111-007-003 Chemicals, peptides, and recombinant proteins Emerald antibody diluent Sigma-Aldrich Cat# 936B-08 Antigen unmasking solution, citrate buffer (1003) Vector Laboratories Cat# H-3300 ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat# H-4000 ImmPress Polymer Reagent anti-Rabbit Vector Laboratories Cat# MP-7451-15 VIP HRP substrate chromogen Vector Laboratories Cat# SK-4600 DPX mounting media Sigma-Aldrich Cat# 6522 Harris hematoxylin Sigma-Aldrich Cat# HHS16 Trizma base Sigma-Aldrich Cat# T1503 NaCl Sigma-Aldrich Cat# S9888 Tween 20 Sigma-Aldrich Cat# P7949 HCL Hydrogen peroxide 30% VWR Cat# 23619.264 PBS (phosphate-buffered saline) (pH 7.4) Gibco Cat# 10010023 DPBS, calcium, magnesium Gibco Cat #14040117 Ethanol absolute (99%) Fisher Scientific Uk Ltd Cat# 10437341 Ammonium hydroxide Sigma-Aldrich Cat# 221228-1L-A Xylene Fisher Scientific Uk Ltd Cat# 10385910 10% neutral buffered formalin CellPath Plc Cat # BAF-0100-25A Dako wash buffer 103 Agilent Cat # S300685-2 Other NanoZoomer S210 Digital slide scanner Hamamatsu Cat# C13239-01 Tefal 6 Liter stainless steel pressure cooker Tefal Cat # 3045384362433 Hot plate Cole-Parmer Cat# 11476508 Microtome Leica Cat# RM2125 RTS Slide jars Simport Cat# M906-12AS Cover slides VWR Cat# 631-0146 IHC slides Thermo Fisher Cat# 12312148 Embedding cassettes Epredia Cat# 16323986 RS PRO Borosilicate Glass 600 mL Beaker RS PRO Cat# 461-1137 Fume hood Fumair Cat# 52716 Staining tray Agar Scientific Cat# AGAR_L4474 Oven (hybridizer hb-1) Techne Cat# 39455-03 Software and algorithms ImageJ FIJI https://imagej.nih.gov/ij/https://fiji.sc/ 1.53f51 QuPath https://qupath.github.io/ 0.2.0m.8 NDP.view2 https://www.hamamatsu.com/us/en/ product/type/U12388-01/index.html 2.7.25 GraphPad https://www.graphpad.com/scientific-software/prism/ 9.0.2 ..
Saline:Article Title: Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD206 Abcam Cat# ab64693; RRID: AB_1523910 PD-L1 clone E1L3N Cell Signaling Technology Cat# 13684; RRID: AB_2687655 AffiniPure Fab Fragment Goat Anti-Rabbit IgG (H+L) Jackson Immunology Cat# 111-007-003 Chemicals, peptides, and recombinant proteins Emerald antibody diluent Sigma-Aldrich Cat# 936B-08 Antigen unmasking solution, citrate buffer (1003) Vector Laboratories Cat# H-3300 ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat# H-4000 ImmPress Polymer Reagent anti-Rabbit Vector Laboratories Cat# MP-7451-15 VIP HRP substrate chromogen Vector Laboratories Cat# SK-4600 DPX mounting media Sigma-Aldrich Cat# 6522 Harris hematoxylin Sigma-Aldrich Cat# HHS16 Trizma base Sigma-Aldrich Cat# T1503 NaCl Sigma-Aldrich Cat# S9888 Tween 20 Sigma-Aldrich Cat# P7949 HCL Hydrogen peroxide 30% VWR Cat# 23619.264 PBS (phosphate-buffered saline) (pH 7.4) Gibco Cat# 10010023 DPBS, calcium, magnesium Gibco Cat #14040117 Ethanol absolute (99%) Fisher Scientific Uk Ltd Cat# 10437341 Ammonium hydroxide Sigma-Aldrich Cat# 221228-1L-A Xylene Fisher Scientific Uk Ltd Cat# 10385910 10% neutral buffered formalin CellPath Plc Cat # BAF-0100-25A Dako wash buffer 103 Agilent Cat # S300685-2 Other NanoZoomer S210 Digital slide scanner Hamamatsu Cat# C13239-01 Tefal 6 Liter stainless steel pressure cooker Tefal Cat # 3045384362433 Hot plate Cole-Parmer Cat# 11476508 Microtome Leica Cat# RM2125 RTS Slide jars Simport Cat# M906-12AS Cover slides VWR Cat# 631-0146 IHC slides Thermo Fisher Cat# 12312148 Embedding cassettes Epredia Cat# 16323986 RS PRO Borosilicate Glass 600 mL Beaker RS PRO Cat# 461-1137 Fume hood Fumair Cat# 52716 Staining tray Agar Scientific Cat# AGAR_L4474 Oven (hybridizer hb-1) Techne Cat# 39455-03 Software and algorithms ImageJ FIJI https://imagej.nih.gov/ij/https://fiji.sc/ 1.53f51 QuPath https://qupath.github.io/ 0.2.0m.8 NDP.view2 https://www.hamamatsu.com/us/en/ product/type/U12388-01/index.html 2.7.25 GraphPad https://www.graphpad.com/scientific-software/prism/ 9.0.2 ..
Immunohistochemistry:Article Title: Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD206 Abcam Cat# ab64693; RRID: AB_1523910 PD-L1 clone E1L3N Cell Signaling Technology Cat# 13684; RRID: AB_2687655 AffiniPure Fab Fragment Goat Anti-Rabbit IgG (H+L) Jackson Immunology Cat# 111-007-003 Chemicals, peptides, and recombinant proteins Emerald antibody diluent Sigma-Aldrich Cat# 936B-08 Antigen unmasking solution, citrate buffer (1003) Vector Laboratories Cat# H-3300 ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat# H-4000 ImmPress Polymer Reagent anti-Rabbit Vector Laboratories Cat# MP-7451-15 VIP HRP substrate chromogen Vector Laboratories Cat# SK-4600 DPX mounting media Sigma-Aldrich Cat# 6522 Harris hematoxylin Sigma-Aldrich Cat# HHS16 Trizma base Sigma-Aldrich Cat# T1503 NaCl Sigma-Aldrich Cat# S9888 Tween 20 Sigma-Aldrich Cat# P7949 HCL Hydrogen peroxide 30% VWR Cat# 23619.264 PBS (phosphate-buffered saline) (pH 7.4) Gibco Cat# 10010023 DPBS, calcium, magnesium Gibco Cat #14040117 Ethanol absolute (99%) Fisher Scientific Uk Ltd Cat# 10437341 Ammonium hydroxide Sigma-Aldrich Cat# 221228-1L-A Xylene Fisher Scientific Uk Ltd Cat# 10385910 10% neutral buffered formalin CellPath Plc Cat # BAF-0100-25A Dako wash buffer 103 Agilent Cat # S300685-2 Other NanoZoomer S210 Digital slide scanner Hamamatsu Cat# C13239-01 Tefal 6 Liter stainless steel pressure cooker Tefal Cat # 3045384362433 Hot plate Cole-Parmer Cat# 11476508 Microtome Leica Cat# RM2125 RTS Slide jars Simport Cat# M906-12AS Cover slides VWR Cat# 631-0146 IHC slides Thermo Fisher Cat# 12312148 Embedding cassettes Epredia Cat# 16323986 RS PRO Borosilicate Glass 600 mL Beaker RS PRO Cat# 461-1137 Fume hood Fumair Cat# 52716 Staining tray Agar Scientific Cat# AGAR_L4474 Oven (hybridizer hb-1) Techne Cat# 39455-03 Software and algorithms ImageJ FIJI https://imagej.nih.gov/ij/https://fiji.sc/ 1.53f51 QuPath https://qupath.github.io/ 0.2.0m.8 NDP.view2 https://www.hamamatsu.com/us/en/ product/type/U12388-01/index.html 2.7.25 GraphPad https://www.graphpad.com/scientific-software/prism/ 9.0.2 ..
Hood:Article Title: Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD206 Abcam Cat# ab64693; RRID: AB_1523910 PD-L1 clone E1L3N Cell Signaling Technology Cat# 13684; RRID: AB_2687655 AffiniPure Fab Fragment Goat Anti-Rabbit IgG (H+L) Jackson Immunology Cat# 111-007-003 Chemicals, peptides, and recombinant proteins Emerald antibody diluent Sigma-Aldrich Cat# 936B-08 Antigen unmasking solution, citrate buffer (1003) Vector Laboratories Cat# H-3300 ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat# H-4000 ImmPress Polymer Reagent anti-Rabbit Vector Laboratories Cat# MP-7451-15 VIP HRP substrate chromogen Vector Laboratories Cat# SK-4600 DPX mounting media Sigma-Aldrich Cat# 6522 Harris hematoxylin Sigma-Aldrich Cat# HHS16 Trizma base Sigma-Aldrich Cat# T1503 NaCl Sigma-Aldrich Cat# S9888 Tween 20 Sigma-Aldrich Cat# P7949 HCL Hydrogen peroxide 30% VWR Cat# 23619.264 PBS (phosphate-buffered saline) (pH 7.4) Gibco Cat# 10010023 DPBS, calcium, magnesium Gibco Cat #14040117 Ethanol absolute (99%) Fisher Scientific Uk Ltd Cat# 10437341 Ammonium hydroxide Sigma-Aldrich Cat# 221228-1L-A Xylene Fisher Scientific Uk Ltd Cat# 10385910 10% neutral buffered formalin CellPath Plc Cat # BAF-0100-25A Dako wash buffer 103 Agilent Cat # S300685-2 Other NanoZoomer S210 Digital slide scanner Hamamatsu Cat# C13239-01 Tefal 6 Liter stainless steel pressure cooker Tefal Cat # 3045384362433 Hot plate Cole-Parmer Cat# 11476508 Microtome Leica Cat# RM2125 RTS Slide jars Simport Cat# M906-12AS Cover slides VWR Cat# 631-0146 IHC slides Thermo Fisher Cat# 12312148 Embedding cassettes Epredia Cat# 16323986 RS PRO Borosilicate Glass 600 mL Beaker RS PRO Cat# 461-1137 Fume hood Fumair Cat# 52716 Staining tray Agar Scientific Cat# AGAR_L4474 Oven (hybridizer hb-1) Techne Cat# 39455-03 Software and algorithms ImageJ FIJI https://imagej.nih.gov/ij/https://fiji.sc/ 1.53f51 QuPath https://qupath.github.io/ 0.2.0m.8 NDP.view2 https://www.hamamatsu.com/us/en/ product/type/U12388-01/index.html 2.7.25 GraphPad https://www.graphpad.com/scientific-software/prism/ 9.0.2 ..
Staining:Article Title: Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD206 Abcam Cat# ab64693; RRID: AB_1523910 PD-L1 clone E1L3N Cell Signaling Technology Cat# 13684; RRID: AB_2687655 AffiniPure Fab Fragment Goat Anti-Rabbit IgG (H+L) Jackson Immunology Cat# 111-007-003 Chemicals, peptides, and recombinant proteins Emerald antibody diluent Sigma-Aldrich Cat# 936B-08 Antigen unmasking solution, citrate buffer (1003) Vector Laboratories Cat# H-3300 ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat# H-4000 ImmPress Polymer Reagent anti-Rabbit Vector Laboratories Cat# MP-7451-15 VIP HRP substrate chromogen Vector Laboratories Cat# SK-4600 DPX mounting media Sigma-Aldrich Cat# 6522 Harris hematoxylin Sigma-Aldrich Cat# HHS16 Trizma base Sigma-Aldrich Cat# T1503 NaCl Sigma-Aldrich Cat# S9888 Tween 20 Sigma-Aldrich Cat# P7949 HCL Hydrogen peroxide 30% VWR Cat# 23619.264 PBS (phosphate-buffered saline) (pH 7.4) Gibco Cat# 10010023 DPBS, calcium, magnesium Gibco Cat #14040117 Ethanol absolute (99%) Fisher Scientific Uk Ltd Cat# 10437341 Ammonium hydroxide Sigma-Aldrich Cat# 221228-1L-A Xylene Fisher Scientific Uk Ltd Cat# 10385910 10% neutral buffered formalin CellPath Plc Cat # BAF-0100-25A Dako wash buffer 103 Agilent Cat # S300685-2 Other NanoZoomer S210 Digital slide scanner Hamamatsu Cat# C13239-01 Tefal 6 Liter stainless steel pressure cooker Tefal Cat # 3045384362433 Hot plate Cole-Parmer Cat# 11476508 Microtome Leica Cat# RM2125 RTS Slide jars Simport Cat# M906-12AS Cover slides VWR Cat# 631-0146 IHC slides Thermo Fisher Cat# 12312148 Embedding cassettes Epredia Cat# 16323986 RS PRO Borosilicate Glass 600 mL Beaker RS PRO Cat# 461-1137 Fume hood Fumair Cat# 52716 Staining tray Agar Scientific Cat# AGAR_L4474 Oven (hybridizer hb-1) Techne Cat# 39455-03 Software and algorithms ImageJ FIJI https://imagej.nih.gov/ij/https://fiji.sc/ 1.53f51 QuPath https://qupath.github.io/ 0.2.0m.8 NDP.view2 https://www.hamamatsu.com/us/en/ product/type/U12388-01/index.html 2.7.25 GraphPad https://www.graphpad.com/scientific-software/prism/ 9.0.2 ..
Software:Article Title: Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CD206 Abcam Cat# ab64693; RRID: AB_1523910 PD-L1 clone E1L3N Cell Signaling Technology Cat# 13684; RRID: AB_2687655 AffiniPure Fab Fragment Goat Anti-Rabbit IgG (H+L) Jackson Immunology Cat# 111-007-003 Chemicals, peptides, and recombinant proteins Emerald antibody diluent Sigma-Aldrich Cat# 936B-08 Antigen unmasking solution, citrate buffer (1003) Vector Laboratories Cat# H-3300 ImmEdge Hydrophobic Barrier PAP Pen Vector Laboratories Cat# H-4000 ImmPress Polymer Reagent anti-Rabbit Vector Laboratories Cat# MP-7451-15 VIP HRP substrate chromogen Vector Laboratories Cat# SK-4600 DPX mounting media Sigma-Aldrich Cat# 6522 Harris hematoxylin Sigma-Aldrich Cat# HHS16 Trizma base Sigma-Aldrich Cat# T1503 NaCl Sigma-Aldrich Cat# S9888 Tween 20 Sigma-Aldrich Cat# P7949 HCL Hydrogen peroxide 30% VWR Cat# 23619.264 PBS (phosphate-buffered saline) (pH 7.4) Gibco Cat# 10010023 DPBS, calcium, magnesium Gibco Cat #14040117 Ethanol absolute (99%) Fisher Scientific Uk Ltd Cat# 10437341 Ammonium hydroxide Sigma-Aldrich Cat# 221228-1L-A Xylene Fisher Scientific Uk Ltd Cat# 10385910 10% neutral buffered formalin CellPath Plc Cat # BAF-0100-25A Dako wash buffer 103 Agilent Cat # S300685-2 Other NanoZoomer S210 Digital slide scanner Hamamatsu Cat# C13239-01 Tefal 6 Liter stainless steel pressure cooker Tefal Cat # 3045384362433 Hot plate Cole-Parmer Cat# 11476508 Microtome Leica Cat# RM2125 RTS Slide jars Simport Cat# M906-12AS Cover slides VWR Cat# 631-0146 IHC slides Thermo Fisher Cat# 12312148 Embedding cassettes Epredia Cat# 16323986 RS PRO Borosilicate Glass 600 mL Beaker RS PRO Cat# 461-1137 Fume hood Fumair Cat# 52716 Staining tray Agar Scientific Cat# AGAR_L4474 Oven (hybridizer hb-1) Techne Cat# 39455-03 Software and algorithms ImageJ FIJI https://imagej.nih.gov/ij/https://fiji.sc/ 1.53f51 QuPath https://qupath.github.io/ 0.2.0m.8 NDP.view2 https://www.hamamatsu.com/us/en/ product/type/U12388-01/index.html 2.7.25 GraphPad https://www.graphpad.com/scientific-software/prism/ 9.0.2 ..
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